Cloning and Mutagenesis Strategies for Large Collagens.
Methods Mol Biol
; 1944: 3-15, 2019.
Article
en En
| MEDLINE
| ID: mdl-30840231
ABSTRACT
The size and relatively high GC content of cDNAs are challenges for efficient targeted engineering of large collagens. There are both basic biological and therapeutic interests in the ability to modify collagens, as this would allow for studies precisely describing interactions of collagens with specific interaction partners, addressing consequences of individual disease-causing mutations, and assessing therapeutic applicability of precision medicine approaches. Using collagen VII as an example, we will here describe a strategy for rapid and simple modification of cDNAs encoding large collagens. The method is flexible and can be used for the creation of point mutations, small or large deletions, and insertion of DNA.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Mutagénesis
/
Clonación Molecular
/
Colágeno Tipo VII
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2019
Tipo del documento:
Article