Helicobacter pylori Vacuolating Cytotoxin A Causes Anorexia and Anxiety via Hypothalamic Urocortin 1 in Mice.
Sci Rep
; 9(1): 6011, 2019 04 12.
Article
en En
| MEDLINE
| ID: mdl-30979915
ABSTRACT
Helicobacter pylori (Hp) infection is related to the pathogenesis of chronic gastric disorders and extragastric diseases. Here, we examined the anorexigenic and anxiogenic effects of Hp vacuolating cytotoxin A (VacA) through activation of hypothalamic urocortin1 (Ucn1). VacA was detected in the hypothalamus after peripheral administration and increased Ucn1 mRNA expression and c-Fos-positive cells in the hypothalamus but not in the nucleus tractus solitarius. c-Fos and Ucn1-double positive cells were detected. CRF1 and CRF2 receptor antagonists suppressed VacA-induced anxiety and anorexia, respectively. VacA activated single paraventricular nucleus neurons and A7r5 cells; this activation was inhibited by phospholipase C (PLC) and protein kinase C (PKC) inhibitors. VacA causes anorexia and anxiety through the intracellular PLC-PKC pathway, migrates across the blood-brain barrier, and activates the Ucn1-CRF receptor axis.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Ansiedad
/
Vacuolas
/
Anorexia
/
Helicobacter pylori
/
Citotoxinas
/
Urocortinas
/
Hipotálamo
Tipo de estudio:
Etiology_studies
Idioma:
En
Revista:
Sci Rep
Año:
2019
Tipo del documento:
Article