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Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Mouse Heart Tissues Using Oroboros 2k-Oxygraph.
Long, Qinqiang; Huang, Lizhen; Huang, Kai; Yang, Qinglin.
Afiliación
  • Long Q; Division of Cardiology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. qqlong@tjh.tjmu.edu.cn.
  • Huang L; School of Basic Medicine, Research Center of Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China.
  • Huang K; Department of Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
  • Yang Q; Division of Cardiology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. qyang@uab.edu.
Methods Mol Biol ; 1966: 237-246, 2019.
Article en En | MEDLINE | ID: mdl-31041752
ABSTRACT
In this chapter, we describe detailed protocols for measuring high-resolution respirometry on mitochondria extracted from adult whole mouse heart using the Oroboros 2k-Oxygraph system. The method provides detailed procedures for the preparation of mitochondria and measurement of high-resolution respirometry in response to various respiration inhibitions. The method described in this chapter could discern the different respiration rate on mitochondria extracted from two spatially distinct mitochondrial subpopulations, subsarcolemmal mitochondria (SSM) and intermyofibrillar mitochondria (IFM). These approaches can easily be translated to other cells and tissues.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Consumo de Oxígeno / Bioquímica / Metabolismo Energético / Mitocondrias Cardíacas / Miocardio Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article
Texto completo
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Consumo de Oxígeno / Bioquímica / Metabolismo Energético / Mitocondrias Cardíacas / Miocardio Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article