Vitexin alleviates interleukin-1ß-induced inflammatory responses in chondrocytes from osteoarthritis patients: Involvement of HIF-1α pathway.
Scand J Immunol
; 90(2): e12773, 2019 Aug.
Article
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| MEDLINE
| ID: mdl-31055848
ABSTRACT
It has been reported that vitexin has anti-inflammatory effects in osteoarthritis (OA) rats. However, the effects of vitexin on interleukins-1ß (IL-1ß)-stimulated OA patient-derived chondrocytes have not been reported. The purpose of this study was to investigate the anti-inflammatory effects of vitexin on IL-1ß-stimulated human osteoarthritis chondrocytes and to reveal the involvement of hypoxia-inducible factor 1α (HIF-1α) pathway. Enzyme-linked immunosorbent assay, quantitative real-time PCR and Western blotting assays were employed. ELISA results demonstrated that the proinflammatory cytokine levels of interleukins-6 (IL-6) and tumour necrosis factor α (TNF-α) in the serum and synovial fluid and HIF-1α level in the synovial fluid were significantly elevated in OA patients compared to normal healthy subjects. Moreover, the Western blotting results indicated that the protein expression of HIF-1α was significantly higher in the cartilage tissues of OA patients. OA patient-derived chondrocytes were stimulated by IL-1ß and treated with different concentration of vitexin for 24 hours. Vitexin showed no cytotoxicity and increased the survival of chondrocytes under IL-1ß stimulation. Vitexin suppressed IL-1ß-induced production of NO and prostaglandin E2 (PGE2 ) in chondrocytes culture. The treatment of vitexin significantly inhibited IL-1ß-induced expressions of proinflammatory cytokine levels of IL-6, TNF-α, matrix metalloproteinase (MMP)-1, MMP-3 and MMP-13. Furthermore, Western blotting results demonstrated that HIF-1α is involved in vitexin's protective effects on IL-1ß-stimulated injuries in OA patient-derived chondrocytes. Our study demonstrates that vitexin alleviates IL-1ß-induced inflammatory responses in chondrocytes from osteoarthritis patients, which may be attributed partly to the inhibition of HIF-1α pathway.
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MEDLINE
Asunto principal:
Osteoartritis
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Condrocitos
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Apigenina
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Subunidad alfa del Factor 1 Inducible por Hipoxia
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Interleucina-1beta
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Antiinflamatorios
Idioma:
En
Revista:
Scand J Immunol
Año:
2019
Tipo del documento:
Article