Rapid liquid chromatography tandem mass spectrometry method for targeted quantitation of human performance metabolites in saliva.
J Chromatogr A
; 1601: 205-213, 2019 Sep 13.
Article
en En
| MEDLINE
| ID: mdl-31060786
ABSTRACT
Saliva is increasingly being targeted for metabolic studies due to its non-invasive collection methods. Tracing levels of certain metabolites within biofluids can provide indications for a myriad of physiological conditions. This study was performed on a panel of eight analytes found in saliva that have shown associations with physiological conditions of human performance, such as stress, inflammation, and circadian rhythm. This dual polarity liquid chromatography tandem mass spectrometric (LCMS/MS) method was developed to accommodate a diverse group of analytes including steroids, alkaloids, and neurotransmitters. Samples collected during field exercises from soldiers were compared to those of civilians and baseline levels of each of these compounds was determined in saliva. Although most analytes showed no significant differences between the two populations, relative cortisol levels were higher for soldiers than for civilians. This developed dual polarity LCMS/MS method can be applied to very diverse groups of salivary analytes simultaneously.
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Base de datos:
MEDLINE
Asunto principal:
Saliva
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Cromatografía Liquida
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Pruebas de Química Clínica
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Doping en los Deportes
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Espectrometría de Masas en Tándem
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Sustancias para Mejorar el Rendimiento
Idioma:
En
Revista:
J Chromatogr A
Año:
2019
Tipo del documento:
Article