In Vitro Evaluation of Candidate Gene Targets for Cancer Therapy.
Methods Mol Biol
; 1974: 21-30, 2019.
Article
en En
| MEDLINE
| ID: mdl-31098992
ABSTRACT
Discovery and development of gene targets for cancer therapeutics are lengthy and highly costly processes. Identification and evaluation of candidate gene targets are of fundamental importance. RNA interference allows candidate genes to be specifically and effectively knocked down in cancer cells. This tool can be easily incorporated into a loss-of-function approach in the initial evaluation of candidate gene targets for cancer treatment prior to moving on to animal studies and clinical trials. This chapter describes a relatively simple and straightforward protocol that makes use of small interfering RNA to achieve knockdown of the candidate gene target and to evaluate the resultant effects on four aspects of cancer cell behavior migration, invasion, proliferation, and adhesion.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Neoplasias de la Mama
/
ARN Interferente Pequeño
/
Interferencia de ARN
/
Biología Molecular
Tipo de estudio:
Guideline
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2019
Tipo del documento:
Article