Metabolic engineering of Escherichia coli for d-pantothenic acid production.
Food Chem
; 294: 267-275, 2019 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-31126462
ABSTRACT
Escherichia coli was engineered to produce d-pantothenic acid via systematic metabolic engineering. Firstly, genes of acetohydroxy acid synthase II, pantothenate synthetase, 3-methyl-2-oxobutanoate hydroxymethyltransferase, 2-dehydropantoate 2-reductase and ketol-acid reductoisomerase were edited in E. coli W3110 with a resulting d-pantothenic acid yield of 0.49â¯g/L. Expressions of valine-pyruvate aminotransferase and branched-chain-amino-acid aminotransferase were then attenuated to decrease the carbon flux in l-valine biosynthetic pathway which is a competing pathway to the d-pantothenic acid biosynthetic pathway, and the yield increased to 1.48â¯g/L. Mutagenesis of pantothenate kinase and deletion of threonine deaminase further increased the production to 1.78â¯g/L. Overexpressions of panC and panB from Corynebacterium glutamicum enhanced the production by 29%. In fed-batch fermentations, strain DPA-9/pTrc99a-panBC(C.G) exhibited a highest d-pantothenic acid yield of 28.45â¯g/L. The findings in this study demonstrate the systematic metabolic engineering in Escherichia coli W3110 would be a promising strategy for industrial production of d-pantothenic acid.
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Base de datos:
MEDLINE
Asunto principal:
Ácido Pantoténico
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Proteínas Bacterianas
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Escherichia coli
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Ingeniería Metabólica
Idioma:
En
Revista:
Food Chem
Año:
2019
Tipo del documento:
Article