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Measurement of Pyridine Nucleotides in Biological Samples Using LC-MS/MS.
Petucci, Chris; Culver, Jeffrey A; Kapoor, Nidhi; Sessions, E Hampton; Divlianska, Daniela; Gardell, Stephen J.
Afiliación
  • Petucci C; Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute, Orlando, FL, USA.
  • Culver JA; Southeast Center for Integrated Metabolomics (SECIM), Gainesville, FL, USA.
  • Kapoor N; Department of Medicine, Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Sessions EH; Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute, Orlando, FL, USA.
  • Divlianska D; Southeast Center for Integrated Metabolomics (SECIM), Gainesville, FL, USA.
  • Gardell SJ; Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute, Orlando, FL, USA.
Methods Mol Biol ; 1996: 61-73, 2019.
Article en En | MEDLINE | ID: mdl-31127548
ABSTRACT
Pyridine nucleotides which include NAD+, NADH, NADP, and NADPH play vital roles in many different biological processes. These metabolites can be accurately quantified in a wide variety of biological samples using LC-MS/MS. The quality and precision of these measurements was enhanced using heavy isotope-labeled internal standards and carefully crafted protocols for sample processing.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Espectrometría de Masas en Tándem / Metabolómica / NAD / NADP Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Espectrometría de Masas en Tándem / Metabolómica / NAD / NADP Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article