Quantification of Melanosome Transfer Using Immunofluorescence Microscopy and Automated Image Analysis.
Methods Mol Biol
; 2109: 55-65, 2020.
Article
en En
| MEDLINE
| ID: mdl-31161578
ABSTRACT
The study of skin pigmentation requires determining the rate of melanin production in melanocytes and quantifying the rate of melanosome transfer to keratinocytes. Here, we describe a method to quantify melanosome transfer using immunofluorescence microscopy coupled with automated image analysis of in vitro human melanocytes and keratinocytes in co-culture. In this method, the number of melanin capped keratinocyte nuclei is quantified.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Queratinocitos
/
Melanosomas
/
Melanocitos
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2020
Tipo del documento:
Article