P(V) Reagents for the Scalable Synthesis of Natural and Modified Nucleoside Triphosphates.
J Am Chem Soc
; 141(34): 13286-13289, 2019 08 28.
Article
en En
| MEDLINE
| ID: mdl-31298849
ABSTRACT
Natural and modified nucleoside triphosphates impact nearly every major aspect of healthcare research from DNA sequencing to drug discovery. However, a scalable synthetic route to these molecules has long been hindered by the need for purification by high performance liquid chromatography (HPLC). Here, we describe a fundamentally different approach that uses a novel P(V) pyrene pyrophosphate reagent to generate derivatives that are purified by silica gel chromatography and converted to the desired compounds on scales vastly exceeding those achievable by HPLC. The power of this approach is demonstrated through the synthesis of a broad range of natural and unnatural nucleoside triphosphates (dNTPs and xNTPs) using protocols that are efficient, inexpensive, and operationally straightforward.
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Base de datos:
MEDLINE
Asunto principal:
Nucleótidos
Idioma:
En
Revista:
J Am Chem Soc
Año:
2019
Tipo del documento:
Article