Auxin-mediated rapid degradation of target proteins in hippocampal neurons.
Neuroreport
; 30(13): 908-913, 2019 09 04.
Article
en En
| MEDLINE
| ID: mdl-31373971
ABSTRACT
Genetic manipulation of protein levels is a promising approach to identify the function of a specific protein in living organisms. Previous studies demonstrated that the auxin-inducible degron strategy provides rapid and reversible degradation of various proteins in fungi and mammalian mitotic cells. In this study, we employed this technology to postmitotic neurons to address whether the auxin-inducible degron system could be applied to the nervous system. Using adeno-associated viruses, we simultaneously introduced enhanced green fluorescent protein (EGFP) fused with an auxin-inducible degron tag and an F-box family protein, TIR1 from Oryza sativa (OsTIR1), into hippocampal neurons from mice. In dissociated hippocampal neurons, EGFP enhanced green fluorescent protein fluorescence signals rapidly decreased when adding a plant hormone, auxin. Furthermore, auxin-induced enhanced green fluorescent protein degradation was also observed in hippocampal acute slices. Taken together, these results open the door for neuroscientists to manipulate protein expression levels by the auxin-inducible degron system in a temporally controlled manner.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Reguladores del Crecimiento de las Plantas
/
Proteolisis
/
Hipocampo
/
Ácidos Indolacéticos
/
Neuronas
Idioma:
En
Revista:
Neuroreport
Asunto de la revista:
NEUROLOGIA
Año:
2019
Tipo del documento:
Article