Molecular characterization of a vitellogenesis-inhibiting hormone (VIH) in the mud crab (Scylla olivacea) and temporal changes in abundances of VIH mRNA transcripts during ovarian maturation and following neurotransmitter administration.
Anim Reprod Sci
; 208: 106122, 2019 Sep.
Article
en En
| MEDLINE
| ID: mdl-31405473
ABSTRACT
The vitellogenesis-inhibiting hormone (VIH), also known as gonad-inhibiting hormone, is a neuropeptide hormone in crustaceans that belongs to the crustacean hyperglycemic hormone (CHH)-family peptide. There is regulation vitellogenesis by VIH during gonad maturation in crustaceans. A full-length Scylla olivacea VIH (Scyol-VIH) was identified through reverse transcription polymerase chain reaction and rapid amplification of cDNA ends. The open reading frame consists of 378 nucleotides, which encodes a 126-amino acid precursor protein, including a 22-residue signal peptide and a 103-amino acid mature peptide in which 6 highly conserved cysteine residues are present. There was expression of the Scyol-VIH gene in immature female Scylla olivacea in the eyestalk, brain and ventral nerve cord. The Scyol-VIH gene expression was localized to the eyestalk X-organ, brain neuronal clusters 6 and 11, and in multiple neuronal clusters of the ventral nerve cord. The relative abundance of Scyol-VIH mRNA transcript in the eyestalk was relatively greater in immature stage females, then decreased as ovarian maturation progressed. Furthermore, eyestalk Scyol-VIH increased after dopamine (5⯵g/g BW) injection. The present research provides fundamental information about Scyol-VIH and its potential effect in controlling reproduction.
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Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Ovario
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ARN Mensajero
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Dopamina
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Braquiuros
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Hormonas de Invertebrados
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Anim Reprod Sci
Año:
2019
Tipo del documento:
Article