RST1 and RIPR connect the cytosolic RNA exosome to the Ski complex in Arabidopsis.
Nat Commun
; 10(1): 3871, 2019 08 27.
Article
en En
| MEDLINE
| ID: mdl-31455787
ABSTRACT
The RNA exosome is a key 3'-5' exoribonuclease with an evolutionarily conserved structure and function. Its cytosolic functions require the co-factors SKI7 and the Ski complex. Here we demonstrate by co-purification experiments that the ARM-repeat protein RESURRECTION1 (RST1) and RST1 INTERACTING PROTEIN (RIPR) connect the cytosolic Arabidopsis RNA exosome to the Ski complex. rst1 and ripr mutants accumulate RNA quality control siRNAs (rqc-siRNAs) produced by the post-transcriptional gene silencing (PTGS) machinery when mRNA degradation is compromised. The small RNA populations observed in rst1 and ripr mutants are also detected in mutants lacking the RRP45B/CER7 core exosome subunit. Thus, molecular and genetic evidence supports a physical and functional link between RST1, RIPR and the RNA exosome. Our data reveal the existence of additional cytosolic exosome co-factors besides the known Ski subunits. RST1 is not restricted to plants, as homologues with a similar domain architecture but unknown function exist in animals, including humans.
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Base de datos:
MEDLINE
Asunto principal:
Arabidopsis
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Proteínas de Arabidopsis
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Interferencia de ARN
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Péptidos y Proteínas de Señalización Intracelular
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Complejo Multienzimático de Ribonucleasas del Exosoma
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Proteínas de la Membrana
Idioma:
En
Revista:
Nat Commun
Asunto de la revista:
BIOLOGIA
/
CIENCIA
Año:
2019
Tipo del documento:
Article