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Characterization of internal ribosome entry sites according to secondary structure analysis to classify border disease virus strains.
Giangaspero, Massimo; Steinbach, Falko; Strong, Rebecca; Decaro, Nicola; Buonavoglia, Canio; Domenis, Lorenzo; Gargano, Pietro; Bailly, Xavier; Apicella, Claudio; Turno, Pasquale.
Afiliación
  • Giangaspero M; Faculty of Veterinary Medicine, University of Teramo, Italy. Electronic address: giangasp@gmail.com.
  • Steinbach F; Mammalian Virology Group, Virology Department, Animal and Plant Health Agency (APHA), United Kingdom.
  • Strong R; Mammalian Virology Group, Virology Department, Animal and Plant Health Agency (APHA), United Kingdom.
  • Decaro N; Faculty of Veterinary Medicine, University of Bari, Italy.
  • Buonavoglia C; Faculty of Veterinary Medicine, University of Bari, Italy.
  • Domenis L; Centro di Referenza Nazionale per le Malattie degli Animali, Selvatici (CeRMAS), Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Quart, Italy.
  • Gargano P; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Catanzaro, Italy.
  • Bailly X; Institut National de la Recherche Agronomique, France.
  • Apicella C; Directorate General of Veterinary Public Health, Food and Nutrition, Ministry of Health, Rome, Italy.
  • Turno P; Directorate General of Veterinary Public Health, Food and Nutrition, Ministry of Health, Rome, Italy.
J Virol Methods ; 275: 113704, 2020 01.
Article en En | MEDLINE | ID: mdl-31518634
ABSTRACT
Applying palindromic nucleotide substitutions (PNS) method, variable loci of the internal ribosome entry site (IRES) secondary structure in the 5' untranslated region (UTR) of Border disease virus sequences were analysed allowing their allocation into ten IRES classes within the species. Sequence characteristics of Turkish and Chinese strains were highly divergent from other genogroups, indicating geographic segregation and micro-evolutive steps within the species. Observed heterogeneity in the BDV species has to be considered for potential implications on diagnostic tests, control and preventive measures.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Filogenia / Genoma Viral / Virus de la Enfermedad de la Frontera / Sitios Internos de Entrada al Ribosoma Idioma: En Revista: J Virol Methods Año: 2020 Tipo del documento: Article
Texto completo
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PubMed Links
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Filogenia / Genoma Viral / Virus de la Enfermedad de la Frontera / Sitios Internos de Entrada al Ribosoma Idioma: En Revista: J Virol Methods Año: 2020 Tipo del documento: Article