Early Diagnosis of Tularemia by Flow Cytometry, Czech Republic, 2003-20151.
Emerg Infect Dis
; 25(10): 1919-1927, 2019 10.
Article
en En
| MEDLINE
| ID: mdl-31538915
ABSTRACT
We retrospectively assessed the utility of a flow cytometry-based test quantifying the percentage of CD3+ T cells with the CD4-/CD8- phenotype for predicting tularemia diagnoses in 64 probable and confirmed tularemia patients treated during 2003-2015 and 342 controls with tularemia-like illnesses treated during 2012-2015 in the Czech Republic. The median percentage of CD3+/CD4-/CD8- T cells in peripheral blood was higher in tularemia patients (19%, 95% CI 17%-22%) than in controls (3%, 95% CI 2%-3%). When we used 8% as the cutoff, this test's sensitivity was 0.953 and specificity 0.895 for distinguishing cases from controls. The CD3+/CD4-/CD8- T cells increased a median of 7 days before tularemia serologic test results became positive. This test supports early presumptive diagnosis of tularemia for clinically suspected cases 7-14 days before diagnosis can be confirmed by serologic testing in regions with low prevalences of tularemia-like illnesses.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Tularemia
/
Citometría de Flujo
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
/
Prognostic_studies
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Risk_factors_studies
/
Screening_studies
País/Región como asunto:
Europa
Idioma:
En
Revista:
Emerg Infect Dis
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
Año:
2019
Tipo del documento:
Article