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Persistent colonization of non-lymphoid tissue-resident macrophages by Stenotrophomonas maltophilia.
Takahashi, Ichiro; Hosomi, Koji; Nagatake, Takahiro; Toubou, Hirokazu; Yamamoto, Daiki; Hayashi, Ikue; Kurashima, Yosuke; Sato, Shintaro; Shibata, Naoko; Goto, Yoshiyuki; Maruyama, Fumito; Nakagawa, Ichiro; Kuwae, Asaomi; Abe, Akio; Kunisawa, Jun; Kiyono, Hiroshi.
Afiliación
  • Takahashi I; Department of Mucosal Immunology, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan.
  • Hosomi K; Laboratory of Vaccine Materials, Center for Vaccine and Adjuvant Research and Laboratory of Gut Environmental System, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki-Osaka, Japan.
  • Nagatake T; Laboratory of Vaccine Materials, Center for Vaccine and Adjuvant Research and Laboratory of Gut Environmental System, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki-Osaka, Japan.
  • Toubou H; Laboratory of Vaccine Materials, Center for Vaccine and Adjuvant Research and Laboratory of Gut Environmental System, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki-Osaka, Japan.
  • Yamamoto D; Department of Mucosal Immunology, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan.
  • Hayashi I; Department of Mucosal Immunology, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan.
  • Kurashima Y; Department of Mucosal Immunology, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan.
  • Sato S; International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
  • Shibata N; International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
  • Goto Y; International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
  • Maruyama F; International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
  • Nakagawa I; Department of Microbiology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
  • Kuwae A; Department of Microbiology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
  • Abe A; Laboratory of Bacterial Infection, Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
  • Kunisawa J; Laboratory of Bacterial Infection, Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
  • Kiyono H; Laboratory of Vaccine Materials, Center for Vaccine and Adjuvant Research and Laboratory of Gut Environmental System, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki-Osaka, Japan.
Int Immunol ; 32(2): 133-141, 2020 02 07.
Article en En | MEDLINE | ID: mdl-31630178
Accumulating evidence has revealed that lymphoid tissue-resident commensal bacteria (e.g. Alcaligenes spp.) survive within dendritic cells. We extended our previous study by investigating microbes that persistently colonize colonic macrophages. 16S rRNA-based metagenome analysis using DNA purified from murine colonic macrophages revealed the presence of Stenotrophomonas maltophilia. The in situ intracellular colonization by S. maltophilia was recapitulated in vitro by using bone marrow-derived macrophages (BMDMs). Co-culture of BMDMs with clinically isolated S. maltophilia led to increased mitochondrial respiration and robust IL-10 production. We further identified a 25-kDa protein encoded by the gene assigned as smlt2713 (recently renamed as SMLT_RS12935) and secreted by S. maltophilia as the factor responsible for enhanced IL-10 production by BMDMs. IL-10 production is critical for maintenance of the symbiotic condition, because intracellular colonization by S. maltophilia was impaired in IL-10-deficient BMDMs, and smlt2713-deficient S. maltophilia failed to persistently colonize IL-10-competent BMDMs. These findings indicate a novel commensal network between colonic macrophages and S. maltophilia that is mediated by IL-10 and smlt2713.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Stenotrophomonas maltophilia / Macrófagos Tipo de estudio: Prognostic_studies Idioma: En Revista: Int Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2020 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Stenotrophomonas maltophilia / Macrófagos Tipo de estudio: Prognostic_studies Idioma: En Revista: Int Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2020 Tipo del documento: Article