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Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering.
Ezhilarasu, Hariharan; Ramalingam, Raghavendra; Dhand, Chetna; Lakshminarayanan, Rajamani; Sadiq, Asif; Gandhimathi, Chinnasamy; Ramakrishna, Seeram; Bay, Boon Huat; Venugopal, Jayarama Reddy; Srinivasan, Dinesh Kumar.
Afiliación
  • Ezhilarasu H; Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117594, Singapore. anthe@nus.edu.sg.
  • Ramalingam R; Department of Mechanical Engineering, Center for Nanofibers & Nanotechnology, Faculty of Engineering, National University of Singapore, Singapore 117576, Singapore. anthe@nus.edu.sg.
  • Dhand C; Department of Mechanical Engineering, Center for Nanofibers & Nanotechnology, Faculty of Engineering, National University of Singapore, Singapore 117576, Singapore. raghavendra8025@gmail.com.
  • Lakshminarayanan R; Anti-Infectives Research Group, Singapore Eye Research Institute, Singapore 169856, Singapore. Chetnachem24@gmail.com.
  • Sadiq A; Anti-Infectives Research Group, Singapore Eye Research Institute, Singapore 169856, Singapore. lakshminarayanan.rajamani@seri.com.sg.
  • Gandhimathi C; Department of Mechanical Engineering, Center for Nanofibers & Nanotechnology, Faculty of Engineering, National University of Singapore, Singapore 117576, Singapore. drancer.asif@gmail.com.
  • Ramakrishna S; Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore. gandhi@tll.org.sg.
  • Bay BH; Department of Mechanical Engineering, Center for Nanofibers & Nanotechnology, Faculty of Engineering, National University of Singapore, Singapore 117576, Singapore. seeram@nus.edu.sg.
  • Venugopal JR; Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117594, Singapore. antbaybh@nus.edu.sg.
  • Srinivasan DK; Department of Mechanical Engineering, Center for Nanofibers & Nanotechnology, Faculty of Engineering, National University of Singapore, Singapore 117576, Singapore. jrvgopal@gmail.com.
Int J Mol Sci ; 20(20)2019 Oct 18.
Article en En | MEDLINE | ID: mdl-31635374
Aloe vera (AV) and tetracycline hydrochloride (TCH) exhibit significant properties such as anti-inflammatory, antioxidant and anti-bacterial activities to facilitate skin tissue engineering. The present study aims to develop poly-ε-caprolactone (PCL)/ AV containing curcumin (CUR), and TCH loaded hybrid nanofibrous scaffolds to validate the synergistic effect on the fibroblast proliferation and antimicrobial activity against Gram-positive and Gram-negative bacteria for wound healing. PCL/AV, PCL/CUR, PCL/AV/CUR and PCL/AV/TCH hybrid nanofibrous mats were fabricated using an electrospinning technique and were characterized for surface morphology, the successful incorporation of active compounds, hydrophilicity and the mechanical property of nanofibers. SEM revealed that there was a decrease in the fiber diameter (ranging from 360 to 770 nm) upon the addition of AV, CUR and TCH in PCL nanofibers, which were randomly oriented with bead free morphology. FTIR spectra of various electrospun samples confirmed the successful incorporation of AV, CUR and TCH into the PCL nanofibers. The fabricated nanofibrous scaffolds possessed mechanical properties within the range of human skin. The biocompatibility of electrospun nanofibrous scaffolds were evaluated on primary human dermal fibroblasts (hDF) by MTS assay, CMFDA, Sirius red and F-actin stainings. The results showed that the fabricated PCL/AV/CUR and PCL/AV/TCH nanofibrous scaffolds were non-toxic and had the potential for wound healing applications. The disc diffusion assay confirmed that the electrospun nanofibrous scaffolds possessed antibacterial activity and provided an effective wound dressing for skin tissue engineering.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Piel / Tetraciclina / Materiales Biocompatibles / Ingeniería de Tejidos / Aloe / Andamios del Tejido / Nanofibras Idioma: En Revista: Int J Mol Sci Año: 2019 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Piel / Tetraciclina / Materiales Biocompatibles / Ingeniería de Tejidos / Aloe / Andamios del Tejido / Nanofibras Idioma: En Revista: Int J Mol Sci Año: 2019 Tipo del documento: Article