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Immune response to abamectin-induced oxidative stress in Chinese mitten crab, Eriocheir sinensis.
Hong, Yuhang; Yin, Hongmei; Huang, Yi; Huang, Qiang; Yang, Xiaozhen.
Afiliación
  • Hong Y; Key Laboratory of Application of Ecology and Environmental Protection in Plateau Wetland of Sichuan, Xichang University, Xichang, 415000, Sichuan Province, China. Electronic address: aquaculturehong@hotmail.com.
  • Yin H; Key Laboratory of Application of Ecology and Environmental Protection in Plateau Wetland of Sichuan, Xichang University, Xichang, 415000, Sichuan Province, China.
  • Huang Y; Key Laboratory of Application of Ecology and Environmental Protection in Plateau Wetland of Sichuan, Xichang University, Xichang, 415000, Sichuan Province, China.
  • Huang Q; Key Laboratory of Application of Ecology and Environmental Protection in Plateau Wetland of Sichuan, Xichang University, Xichang, 415000, Sichuan Province, China.
  • Yang X; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Engineering Research Center of Agriculture, Shanghai Ocean University, 999 Huchenghuan Road, Lingang New District, Shanghai, 201306, China.
Ecotoxicol Environ Saf ; 188: 109889, 2020 Jan 30.
Article en En | MEDLINE | ID: mdl-31704331
ABSTRACT
It is known that abamectin (ABM) inflicts oxidative damage on aquatic animals; however, knowledge about the immune response under pesticide-induced oxidative stress is incomplete. In the present study, several cellular and humoral immune parameters, including total haemocyte counts (THC), lysosomal membrane stability (LMS), activities of acid phosphatase (ACP), alkaline phosphatase (AKP) and lysozyme (LZM) were investigated to reveal the effects of ABM exposure on the immune defence mechanisms of the important freshwater crab, Erocheir sinensis. According to the results, a significant increase of THC was found in low concentration groups (0.03 and 0.06 mg/L), while dramatic decreases occurred in high concentration groups (0.12 and 0.24 mg/L) after 96 h of exposure. We also detected significant increases of reactive oxygen species (ROS) in haemocytes at 0.12 and 0.24 mg/L, and there was a dose- and time-dependent decrease of lysosomal membrane stability. These results suggest that the excessive generation of ROS induced by ABM may be leading the massive collapse of lysosomal membrane, which in turn may be causing the sharp drop of haemocyte counts in E. sinensis. The increase of hydrolytic enzymes ACP and AKP at low concentrations and the decrease at high concentrations also indicate an immune response associated with haemocytes status under stress. However, activities of LZM decreased significantly. After injection of Aeromonas hydrophil, mortalities increased under exposure to ABM and were positively related to ABM concentration. These results confirm that ABM exposure has the ability to impair immune defence and result in the host's susceptibility to pathogens.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Contaminantes Químicos del Agua / Ivermectina / Estrés Oxidativo / Braquiuros / Insecticidas Idioma: En Revista: Ecotoxicol Environ Saf Año: 2020 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Contaminantes Químicos del Agua / Ivermectina / Estrés Oxidativo / Braquiuros / Insecticidas Idioma: En Revista: Ecotoxicol Environ Saf Año: 2020 Tipo del documento: Article