Quantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study, 2011-2018.
Sci Rep
; 9(1): 16675, 2019 11 13.
Article
en En
| MEDLINE
| ID: mdl-31723144
ABSTRACT
Household contacts (HHC) of leprosy patients exhibit high-risk of developing leprosy and contact tracing is helpful for early diagnosis. From 2011 to 2018,2,437 HHC were examined in a clinic in Rio de Janeiro, Brazil and 16S qPCR was used for diagnosis and monitoring of contacts. Fifty-four HHCs were clinically diagnosed with leprosy at intake. Another 25 exhibited leprosy-like skin lesions at intake, 8 of which were confirmed as having leprosy (50% of which were qPCR positive) and 17 of which were diagnosed with other skin diseases (6% qPCR positive). In skin biopsies, qPCR presented a sensitivity of 0.50 and specificity of 0.94. Furthermore, 955 healthy HHCs were followed-up for at least 3 years and skin scrapings were collected from earlobes for qPCR detection. Positive qPCR indicated a non-significant relative risk of 2.52 of developing the disease. During follow-up, those who progressed towards leprosy exhibited 20% qPCR positivity, compared to 9% of those who remained healthy. Disease-free survival rates indicated that age had a significant impact on disease progression, where patients over 60 had a greater chance of developing leprosy [HR = 32.4 (3.6-290.3)]. Contact tracing combined with qPCR may assist in early diagnosis and age is a risk factor for leprosy progression.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
ADN Bacteriano
/
ADN Ribosómico
/
Composición Familiar
/
Trazado de Contacto
/
Reacción en Cadena en Tiempo Real de la Polimerasa
/
Lepra
/
Mycobacterium leprae
Tipo de estudio:
Diagnostic_studies
/
Etiology_studies
/
Incidence_studies
/
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
/
Screening_studies
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Sci Rep
Año:
2019
Tipo del documento:
Article