Enhancing membrane modulus of giant unilamellar lipid vesicles by lateral co-assembly of amphiphilic triblock copolymers.
J Colloid Interface Sci
; 561: 318-326, 2020 Mar 01.
Article
en En
| MEDLINE
| ID: mdl-31740134
ABSTRACT
We report a facile, but robust approach to fabricate structurally stable giant unilamellar vesicles (GUVs), on which a 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) bilayer membrane was made rigid by introducing amphiphilic block polymers. Particularly, we found that lateral co-assembly of an amphiphilic triblock copolymer (ATC) structured with a hydrophobic middle block and long molecular weight (20â¯Kâ¯g/mol) hydrophilic end blocks remarkably enhanced the stretching modulus (k) of GUVs. When the membrane composition was optimized, the k value of ATC-hybridized GUVs increased to 6.2â¯×â¯108â¯Pa, which was approximately 10-fold higher than that of DPPC GUVs, thus leading to a much longer half-life. Moreover, we demonstrated that our ATC-hybridized GUVs enabled development of a fascinating vesicular model, which shows great potential as a structurally stable cell membrane mimic.
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Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Polímeros
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1,2-Dipalmitoilfosfatidilcolina
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Liposomas Unilamelares
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Membrana Dobles de Lípidos
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Liposomas
Idioma:
En
Revista:
J Colloid Interface Sci
Año:
2020
Tipo del documento:
Article