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Eicosapentaenoic acid prevents inflammation induced by acute cerebral infarction through inhibition of NLRP3 inflammasome activation.
Mo, Zhihuai; Tang, Chaogang; Li, Huiqing; Lei, Junjie; Zhu, Lingjuan; Kou, Li; Li, Hao; Luo, Shijian; Li, Chunyi; Chen, Wenli; Zhang, Lei.
Afiliación
  • Mo Z; Department of Neurology, The Fifth Affiliated Hospital of Sun Yat-sen University, 519000, Guangdong, China.
  • Tang C; Department of Neurology, The Fifth Affiliated Hospital of Sun Yat-sen University, 519000, Guangdong, China; Department of Neurology, Maoming People's Hospital, 525000, Guangdong, China.
  • Li H; Department of Neurology, The Fifth Affiliated Hospital of Sun Yat-sen University, 519000, Guangdong, China.
  • Lei J; Department of Neurology, The Fifth Affiliated Hospital of Sun Yat-sen University, 519000, Guangdong, China.
  • Zhu L; Department of Neurology, The Fifth Affiliated Hospital of Sun Yat-sen University, 519000, Guangdong, China.
  • Kou L; Department of Neurology, The Fifth Affiliated Hospital of Sun Yat-sen University, 519000, Guangdong, China.
  • Li H; Department of Neurology, The Fifth Affiliated Hospital of Sun Yat-sen University, 519000, Guangdong, China; Department of Neurology, Maoming People's Hospital, 525000, Guangdong, China.
  • Luo S; Department of Neurology, The Fifth Affiliated Hospital of Sun Yat-sen University, 519000, Guangdong, China.
  • Li C; Department of Neurology, The Fifth Affiliated Hospital of Sun Yat-sen University, 519000, Guangdong, China.
  • Chen W; Department of Pharmacology, The Fifth Affiliated Hospital of Sun Yat-sen University, 519000, Guangdong, China. Electronic address: chenwenl@mail3.sysu.edu.cn.
  • Zhang L; Department of Neurology, The Fifth Affiliated Hospital of Sun Yat-sen University, 519000, Guangdong, China. Electronic address: zhangl92@sysu.edu.cn.
Life Sci ; 242: 117133, 2020 Feb 01.
Article en En | MEDLINE | ID: mdl-31830477
OBJECTIVE: Acute cerebral infarction (ACI) is the most common type of acute cerebrovascular diseases resulting in high rate of death and disability. Numerous evidences show that inflammation is the leading cause of ischemic brain injury, thus anti-inflammatory therapy is an attractive candidate for ischemic brain damage. Eicosapentaenoic acid (EPA) exerts anti-inflammatory activity in lots of human inflammatory diseases, whereas its effect in ACI is left to elucidate. METHOD: Nlpr3-/- mice, Gpr40-/-; Gpr120-/- mice and mice with right middle cerebral artery occlusion (MCAO) were used to detect NLR family pyrin domain containing 3 (NLRP3) inflammasome activation by Western Blot and the release of proinflammatory cytokines by ELISA. To estimate the acute ischemic condition in vitro, oxygen-glucose deprivation (OGD) was induced in BV2 microglia cells. Transfection of the shRNA targeting GPR40 and GPR120 mRNA into BV2 cells was also assessed. Apoptosis in ischemic cerebral tissues and BV2 cells was detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay and flow cytometry. RESULT: Here we show that EPA suppresses ACI-induced inflammatory responses through blocking NLRP3 inflammasome activation. In addition, EPA inhibits NLRP3 inflammasome activation through G protein-coupled receptor 40 (GPR40) and GPR120. Importantly, EPA ameliorates ACI-induced apoptosis. CONCLUSION: EPA exerts beneficial effect on ACI-induced inflammation through blocking NLRP3 inflammasome activation by GPR40 and GPR120. Our findings suggest the potential clinical use of EPA in ACI.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Infarto Cerebral / Ácido Eicosapentaenoico / Inflamasomas / Proteína con Dominio Pirina 3 de la Familia NLR / Inflamación Tipo de estudio: Etiology_studies / Prognostic_studies Idioma: En Revista: Life Sci Año: 2020 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Infarto Cerebral / Ácido Eicosapentaenoico / Inflamasomas / Proteína con Dominio Pirina 3 de la Familia NLR / Inflamación Tipo de estudio: Etiology_studies / Prognostic_studies Idioma: En Revista: Life Sci Año: 2020 Tipo del documento: Article