Structural and Biomechanical Characterization of a Scaffold-Free Skin Equivalent Model via Biophysical Methods.
Skin Pharmacol Physiol
; 33(1): 17-29, 2020.
Article
en En
| MEDLINE
| ID: mdl-31852002
ABSTRACT
AIMS:
Among in vitro skin models, the scaffold-free skin equivalent (SFSE), without exogenous material, is interesting for pharmacotoxicological studies. Our aim was to adapt in vivo biophysical methods to study the structure, thickness, and extracellular matrix of our in vitro model without any chemical fixation needed as for histology.METHODS:
We evaluated 3 batches of SFSE and characterized them by histology, transmission electron microscopy (TEM), and immunofluorescence. In parallel, we investigated 3 biophysical methods classically used for in vivo evaluation, optical coherence tomography (OCT), and laser scanning microscopy (LSM) imaging devices as well as the cutometer suction to study the biomechanical properties.RESULTS:
OCT allowed the evaluation of SFSE total thickness and its different compartments. LSM has a greater resolution enabling an evaluation at the cell scale and the orientation of collagen fibers. The viscoelasticity measurement by cutometry was possible on our thin skin model and might be linked with mature collagen bundles visible in TEM and LSM and with elastic fibers seen in immunofluorescence.CONCLUSION:
Our data demonstrated the simplicity and sensitivity of these different in vivo biophysical devices on our thin skin model. These noninvasive tools allow to study the morphology and the biomechanics of in vitro models.Palabras clave
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Base de datos:
MEDLINE
Asunto principal:
Piel
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Ingeniería de Tejidos
Idioma:
En
Revista:
Skin Pharmacol Physiol
Asunto de la revista:
DERMATOLOGIA
/
FARMACOLOGIA
/
FISIOLOGIA
Año:
2020
Tipo del documento:
Article