In vitro assays for effector T cell functions and activity of immunomodulatory antibodies.
Methods Enzymol
; 631: 43-59, 2020.
Article
en En
| MEDLINE
| ID: mdl-31948562
ABSTRACT
The recent clinical success of cancer immunotherapy with checkpoint blockade has led to renewed interest into the development of immune modulatory agents with the capacity to activate anti-tumor T cell responses. Standardization of optimized in vitro assays for efficient assessment of immune function of such new drugs is thus needed to facilitate clinical development of the optimal drug candidates. Here, we describe an optimized version of T cell suppression assay designed to test the effect of immunomodulatory agents on T cell function and activation. We apply this assay to investigate the agonist activity of the T cell co-stimulatory molecule glucocorticoid-induced TNFR-related protein (GITR). We detail a protocol for concurrent assessment of multiple levels of T cell functional modulation upon GITR engagement, including T cell priming, activation and effector function, in a single assay. As human GITR agonist antibodies are currently under development, availability of standardized cell-based functional assays of GITR agonism is instrumental to translate anti-GITR therapy into the clinical setting.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Activación de Linfocitos
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Linfocitos T
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Técnicas Inmunológicas
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Proteína Relacionada con TNFR Inducida por Glucocorticoide
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Inmunomodulación
Tipo de estudio:
Guideline
Idioma:
En
Revista:
Methods Enzymol
Año:
2020
Tipo del documento:
Article