Lipopolysaccharide (LPS) inhibits ectopic bone formation induced by bone morphogenetic protein-2 and TGF-ß1 through IL-1ß production.
J Oral Biosci
; 62(1): 44-51, 2020 03.
Article
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| MEDLINE
| ID: mdl-31987892
ABSTRACT
OBJECTIVES:
In order to gain new insight into bacterial infection during bone-regenerative treatment using bone morphogenetic proteins (BMPs), we examined the effects of lipopolysaccharide (LPS) on ectopic bone formation induced by BMP-2 and transforming growth factor (TGF)-ß1 in mice.METHODS:
We implanted collagen sponges containing BMP-2, TGF-ß1, and various amounts of LPS into mouse muscle tissues. Lump-like masses in which ectopic bones developed in mice were processed for microcomputed tomography, DNA microarray, reverse-transcription PCR, and histological analyses.RESULTS:
LPS treatment caused a dose-dependent reduction in the volume of ectopic bone. The total volume of ectopic bone induced by BMP-2 + TGF-ß1 treatment was reduced by more than 75% in the presence of LPS. Histological analysis of the ectopic bone tissues revealed a significant reduction in total bone volume and bone volume/total volume in response to LPS. LPS treatment significantly increased the osteoblast number and osteoid volume, while the osteoclast number did not change. Since LPS induced production of TNF-α and IL-1ß in lump-like masses, we implanted collagen sponges containing BMP-2 and TGF-ß1 with or without LPS into TNF-α- or IL-1α/ß-deficient mice. LPS treatment reduced the volume of ectopic bones in TNF-α-deficient mice but not in IL-1α/ß-deficient mice. Furthermore, collagen sponges containing IL-1ß reduced ectopic bone formation by BMP-2 and TGF-ß1 in wild-type mice to the same extent as LPS treatment did.CONCLUSIONS:
LPS suppresses the ectopic bone formation induced by BMP-2 and TGF-ß1 through IL-1ß production.Palabras clave
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Base de datos:
MEDLINE
Asunto principal:
Osificación Heterotópica
/
Factor de Crecimiento Transformador beta1
Idioma:
En
Revista:
J Oral Biosci
Año:
2020
Tipo del documento:
Article