Impact of CRISPR interference on strain development in biotechnology.
Biotechnol Appl Biochem
; 67(1): 7-21, 2020 Jan.
Article
en En
| MEDLINE
| ID: mdl-32064678
ABSTRACT
Genetic perturbation systems are of great interest to redirect metabolic fluxes for value-added production, as well as genetic screening for the development of new drugs, or to identify new targets for biotechnological applications. Here, we review CRISPR interference (CRISPRi), a method for gene expression using a catalytically inactive version of the CRISPR-associated protein 9 (dCas9) of the widely applied CRISPR-Cas9 genome editing system. In combination with the appropriate sgRNA, dCas9 binds to specific DNA sequences without causing double-stranded DNA breakage but interfering with transcription initiation or elongation. Besides manifold uses to interrogate the physiology of a bacterial cell, CRISPRi is used in applications for metabolic engineering and strain development in industrial biotechnology. Albeit in its infancy, CRISPRi has already delivered the first success stories; however, we also analyze limitations of the CRISPRi system and give future perspectives.
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Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Biotecnología
/
Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas
Idioma:
En
Revista:
Biotechnol Appl Biochem
Asunto de la revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Año:
2020
Tipo del documento:
Article