Your browser doesn't support javascript.
loading
Betaine-loaded CaCO3 microparticles improve survival of vitrified feline preantral follicles through higher mitochondrial activity and decreased reactive oxygen species.
Brito, D C C; Domingues, S F S; Rodrigues, A P R; Silva, L M; Alves, K A; Wu, X; Francisco, T S; Barroso Neto, I L; Freire, V N; Figueiredo, J R; Pieczarka, J C; Santos, R R.
Afiliación
  • Brito DCC; Laboratory of Cytogenetics, Center for Advanced Studies in Biodiversity, Biological Sciences Institute, Federal University of Pará, Av. Perimetral, 2-224 - Guamá, 66077-830, Belém-PA, Brazil; and Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Av. Perimetral, 2-224 - Guam
  • Domingues SFS; Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Av. Perimetral, 2-224 - Guamá, 66077-830, Belém-PA, Brazil.
  • Rodrigues APR; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles, Faculty of Veterinary Medicine, Ceará State University, Av. Dr. Silas Munguba, 1700 - Itaperi, 60714-903, Fortaleza-CE, Brazil.
  • Silva LM; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles, Faculty of Veterinary Medicine, Ceará State University, Av. Dr. Silas Munguba, 1700 - Itaperi, 60714-903, Fortaleza-CE, Brazil.
  • Alves KA; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles, Faculty of Veterinary Medicine, Ceará State University, Av. Dr. Silas Munguba, 1700 - Itaperi, 60714-903, Fortaleza-CE, Brazil.
  • Wu X; Hubert Department of Global Health, Rollins School of Public Health, Emory University, 1518 Clifton Rd, Atlanta, GA 30322, USA; and Department of Infectious Disease, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Yuhangtang Road 866, Hangzhou, Zhejiang, PR China.
  • Francisco TS; Department of Physics, Federal University of Ceará, Av. da Universidade, 2853 - Benfica, 60020-181, Fortaleza - CE, Brazil; and Department of Chemistry, State University of Vale do Acarau, Av. da Universidade, 850 - Jerônimo de Medeiros Prado, Sobral - CE, 62010-295, Brazil.
  • Barroso Neto IL; Department of Physics, Federal University of Ceará, Av. da Universidade, 2853 - Benfica, 60020-181, Fortaleza - CE, Brazil.
  • Freire VN; Department of Physics, Federal University of Ceará, Av. da Universidade, 2853 - Benfica, 60020-181, Fortaleza - CE, Brazil.
  • Figueiredo JR; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles, Faculty of Veterinary Medicine, Ceará State University, Av. Dr. Silas Munguba, 1700 - Itaperi, 60714-903, Fortaleza-CE, Brazil.
  • Pieczarka JC; Laboratory of Cytogenetics, Center for Advanced Studies in Biodiversity, Biological Sciences Institute, Federal University of Pará, Av. Perimetral, 2-224 - Guamá, 66077-830, Belém-PA, Brazil.
  • Santos RR; Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Av. Perimetral, 2-224 - Guamá, 66077-830, Belém-PA, Brazil.
Reprod Fertil Dev ; 32(5): 531-537, 2020 Mar.
Article en En | MEDLINE | ID: mdl-32087765
ABSTRACT
Ovary fragments from six sexually mature cats were vitrified in the presence or absence of betaine or ascorbic acid, loaded (7.4 or 74µM betaine; 20 or 200µM ascorbic acid) or not (1mM betaine or 0.3mM ascorbic acid) into CaCO3 microparticles, and assessed for follicular morphology, oxidative stress and mitochondrial activity Feline ovarian tissue was successfully preserved after vitrification in the presence of 74µM betaine loaded in CaCO3 microparticles, as confirmed by morphological analysis and the density of preantral follicles and stromal cells, as well as by the increased mitochondrial activity and decreased production of reactive oxygen species.
Asunto(s)
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Betaína / Carbonato de Calcio / Criopreservación / Especies Reactivas de Oxígeno / Folículo Ovárico / Mitocondrias Idioma: En Revista: Reprod Fertil Dev Asunto de la revista: MEDICINA REPRODUTIVA Año: 2020 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Betaína / Carbonato de Calcio / Criopreservación / Especies Reactivas de Oxígeno / Folículo Ovárico / Mitocondrias Idioma: En Revista: Reprod Fertil Dev Asunto de la revista: MEDICINA REPRODUTIVA Año: 2020 Tipo del documento: Article