Multicolor Labeling and Tracing of Pancreatic Beta-Cell Proliferation in Zebrafish.
Methods Mol Biol
; 2128: 159-179, 2020.
Article
en En
| MEDLINE
| ID: mdl-32180193
ABSTRACT
During embryogenesis, beta-cells arise from the dorsal and ventral bud originating in the endoderm germ layer. As the animal develops to adulthood, the beta-cell mass dramatically increases. The expansion of the beta-cell population is driven by cell division among the embryonic beta-cells and supplanted by neogenesis from post-embryonic progenitors. Here, we describe a protocol for multicolor clonal analysis in zebrafish to define the contribution of individual embryonic beta-cells to the increase in cell numbers. This technique provides insights into the proliferative history of individual beta-cells in an islet. This insight helps in defining the replicative heterogeneity among individual beta-cells during development. Additionally, the ability to discriminate individual cells based on unique color signatures helps quantify the volume occupied by beta-cells and define the contribution of cellular size to the beta-cell mass.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Coloración y Etiquetado
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Procesamiento de Imagen Asistido por Computador
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Microscopía Confocal
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Proliferación Celular
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Células Secretoras de Insulina
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Rastreo Celular
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2020
Tipo del documento:
Article