Dynamic light scattering biosensing based on analyte-induced inhibition of nanoparticle aggregation.
Anal Bioanal Chem
; 412(14): 3423-3431, 2020 May.
Article
en En
| MEDLINE
| ID: mdl-32279165
ABSTRACT
A new approach to direct quantitative detection of small molecules (haptens) by dynamic light scattering biosensing is presented. The proposed technique implements a homogeneous competitive immunoassay and is based on optical detection of specific inhibition of nanoparticle aggregation induced by the analyte in a sample. The technique performance was tested both in buffer and milk for detection of chloramphenicol - antibiotic relevant to food safety diagnostics. Good specificity, sensitivity (LOD in milk is 2.4 ng/ml), precision (4.0 ± 1.2%), ruggedness (8.3%), and 96% recovery in conjunction with a record wide dynamic range (3 orders of magnitude) of the nanosensing technique were demonstrated. Such characteristics complemented by the assay simplicity (no washing step) and a short assay time make the approach attractive for application as an analytical platform for point-of-care and field-oriented diagnostics. Graphical abstract.
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Base de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
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Cloranfenicol
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Nanopartículas del Metal
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Anticuerpos Inmovilizados
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Dispersión Dinámica de Luz
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Antibacterianos
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2020
Tipo del documento:
Article