A SERS quenching method for the sensitive determination of insulin.
Drug Test Anal
; 13(5): 1048-1053, 2021 May.
Article
en En
| MEDLINE
| ID: mdl-32311837
ABSTRACT
In this work, we utilise the disulphide bond structure of insulin and a new benzothiazole Raman probe for the detection of human insulin using surface-enhanced Raman spectroscopy (SERS). The disulphide bond structure of the insulin was reduced to generate free sulfhydryl terminal groups. When reacted with benzothiazole-functionalised gold nanoparticles, the reduced protein desorbs the Raman probe and causes its Raman signal intensity to quench. Using this approach, insulin was quantified in the concentration range of 1 × 10-14 -1 × 10-8 M by SERS quenching. The limit of quantification of insulin by the SERS quenching method was found to be 1 × 10-14 M (0.01 pM or 58 pg/L), which satisfies the requirements for monitoring its blood concentration in patients. Because many proteins and peptides have disulphide bonds in their molecular structures, the new SERS quenching method has a strong potential for the rapid determination of ultralow concentrations of proteins in formulations and biological fluids.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Espectrometría Raman
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Disulfuros
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Insulina
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Drug Test Anal
Asunto de la revista:
FARMACOLOGIA
Año:
2021
Tipo del documento:
Article