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Cell-modified bioprinted microspheres for vascular regeneration.
Shen, Jian; Ji, Yongli; Xie, Mingjun; Zhao, Haiming; Xuan, Wanling; Yin, Li; Yu, Xiaohua; Xu, Fangfang; Su, Shengan; Nie, Jing; Xie, Yao; Gao, Qing; Ma, Hong; Ke, Xueying; Shi, Zhenyu; Fu, Jianzhong; Liu, Zhenjie; He, Yong; Xiang, Meixiang.
Afiliación
  • Shen J; Department of Cardiology, Cardiovascular Key Lab of Zhejiang Province, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310000, China.
  • Ji Y; Department of Cardiology, Cardiovascular Key Lab of Zhejiang Province, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310000, China.
  • Xie M; State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, and Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou 310000, China.
  • Zhao H; State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, and Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou 310000, China.
  • Xuan W; Vascular Biology Center, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA.
  • Yin L; Department of Vascular Surgery, Second Affiliated Hospital of Zhejiang University School of Medicine, China.
  • Yu X; Hangzhou Huamai Medical Devices Co., Ltd., Hangzhou, Zhejiang 310053, China.
  • Xu F; Department of Radiology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, China.
  • Su S; Department of Cardiology, Cardiovascular Key Lab of Zhejiang Province, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310000, China.
  • Nie J; State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, and Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou 310000, China.
  • Xie Y; Department of Cardiology, Cardiovascular Key Lab of Zhejiang Province, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310000, China.
  • Gao Q; State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, and Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou 310000, China.
  • Ma H; Department of Cardiology, Cardiovascular Key Lab of Zhejiang Province, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310000, China.
  • Ke X; Department of Vascular Surgery, Second Affiliated Hospital of Zhejiang University School of Medicine, China.
  • Shi Z; Department of Vascular Surgery, Zhongshan Hospital, Fudan University, Shanghai 200000, China.
  • Fu J; State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, and Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou 310000, China.
  • Liu Z; Department of Vascular Surgery, Second Affiliated Hospital of Zhejiang University School of Medicine, China. Electronic address: lawson4001@zju.edu.cn.
  • He Y; State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, and Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou 310000, China. Electronic address: yonghe@zju.edu.cn.
  • Xiang M; Department of Cardiology, Cardiovascular Key Lab of Zhejiang Province, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310000, China. Electronic address: xiangmx@zju.edu.cn.
Mater Sci Eng C Mater Biol Appl ; 112: 110896, 2020 Jul.
Article en En | MEDLINE | ID: mdl-32409053
Cell therapy is a promising strategy in which living cells or cellular materials are delivered to treat a variety of diseases. Here, we developed an electrospray bioprinting method to rapidly generate cell-laden hydrogel microspheres, which limit the migration of the captured cells and provide an immunologically privileged microenvironment for cell survival in vivo. Currently, therapeutic angiogenesis aims to induce collateral vessel formation after limb ischemia. However, the clinical application of gene and cell therapy has been impeded by concerns regarding its inefficacy, as well as the associated risk of immunogenicity and oncogenicity. In this study, hydrogel microspheres encapsulating VEGF-overexpressing HEK293T cells showed good safety via subcutaneously injecting into male C57BL/6 mice. In addition, these cell-modified microspheres effectively promoted angiogenesis in a mouse hind-limb ischemia model. Therefore, we demonstrated the great therapeutic potential of this approach to induce angiogenesis in limb ischemia, indicating that bioprinting has a bright future in cell therapy.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Materiales Biocompatibles / Neovascularización Fisiológica / Microesferas Idioma: En Revista: Mater Sci Eng C Mater Biol Appl Año: 2020 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Materiales Biocompatibles / Neovascularización Fisiológica / Microesferas Idioma: En Revista: Mater Sci Eng C Mater Biol Appl Año: 2020 Tipo del documento: Article