Substrate specificity of the galactokinase from the human gut symbiont Akkermansia muciniphila ATCC BAA-835.
Enzyme Microb Technol
; 139: 109568, 2020 Sep.
Article
en En
| MEDLINE
| ID: mdl-32732027
ABSTRACT
Galactokinases, which catalyze the phosphorylation of galactose and possible other monosaccharides, can provide an activated sugar donor to synthesize sugar-containing molecules. In this study, a novel galactokinase from human gut symbiont Akkermansia muciniphila ATCC BAA-835 (GalKAmu) was expressed and characterized. GalKAmu displayed broad substrate tolerance, with catalytic activity towards Gal (100 %), GalN (100 %), GalA (20.2 %), Glc (52.5 %), GlcNAc (15.5 %), Xyl (<5%), ManNAc (58 %), ManF (37.4 %) and l-Glc (80 %). Most interestingly, this was the first GalK isoform which can tolerate ManNAc. Thus, our characterization of GalKAmu broadens the substrate selection of galactokinases.
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1
Base de datos:
MEDLINE
Asunto principal:
Simbiosis
/
Microbioma Gastrointestinal
/
Galactoquinasa
Idioma:
En
Revista:
Enzyme Microb Technol
Año:
2020
Tipo del documento:
Article