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In Vitro and In Vivo Evaluation of Fluorescently Labeled Borocaptate-Containing Liposomes.
Kanygin, Vladimir; Zaboronok, Alexander; Taskaeva, Iuliia; Zavjalov, Evgenii; Mukhamadiyarov, Rinat; Kichigin, Aleksandr; Kasatova, Anna; Razumov, Ivan; Sibirtsev, Roman; Mathis, Bryan J.
Afiliación
  • Kanygin V; Laboratory of Medical and Biological Problems of BNCT, Novosibirsk State University, Novosibirsk, Russian Federation.
  • Zaboronok A; Laboratory of Medical and Biological Problems of BNCT, Novosibirsk State University, Novosibirsk, Russian Federation. a.zaboronok@md.tsukuba.ac.jp.
  • Taskaeva I; Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan. a.zaboronok@md.tsukuba.ac.jp.
  • Zavjalov E; Department of Neurosurgery, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan. a.zaboronok@md.tsukuba.ac.jp.
  • Mukhamadiyarov R; Laboratory of Ultrastructural Research, Research Institute of Clinical and Experimental Lymphology - Branch of the Institute of Cytology and Genetics SB RAS, Novosibirsk, Russian Federation.
  • Kichigin A; Laboratory of BNCT, Novosibirsk State University, Novosibirsk, Russian Federation.
  • Kasatova A; Budker Institute of Nuclear Physics, Novosibirsk, Russian Federation.
  • Razumov I; Laboratory of Medical and Biological Problems of BNCT, Novosibirsk State University, Novosibirsk, Russian Federation.
  • Sibirtsev R; Center for Genetic Resources of Laboratory Animals, Institute of Cytology and Genetics SB RAS, Novosibirsk, Russian Federation.
  • Mathis BJ; Laboratory of Medical and Biological Problems of BNCT, Novosibirsk State University, Novosibirsk, Russian Federation.
J Fluoresc ; 31(1): 73-83, 2021 Jan.
Article en En | MEDLINE | ID: mdl-33078252
ABSTRACT
Boron neutron capture therapy (BNCT), a binary cancer therapeutic modality, has moved to a new phase since development of accelerator-based neutron sources and establishment of BNCT centers in Finland and Japan. That stimulated efforts for better boron delivery agent development. As liposomes have shown effective boron delivery properties and sufficient tumor retention, fluorescent liposome labelling may serve as a rapid method to study initial ability of newly synthesized liposomes to be captured by tumor cells prior to experiments on boron accumulation and neutron irradiation. In this work, we studied the accumulation and biodistribution of pegylated liposomes with encapsulated borocaptate (BSH) and a fluorescent label (Nile Red) in U87 (human glioblastoma), SW-620 (human colon carcinoma), SK-MEL-28 (human melanoma), FetMSC (mesenchymal human embryo stem cells), and EMBR (primary embryocytes) cell lines as well as an orthotopic xenograft model of U87 glioma in SCID mice. Results indicate that fluorescent microscopy is effective at determining the intracellular localization of the liposomes using a fluorescent label. The synthesized, pegylated liposomes showed higher accumulation in tumors compared to normal cells, with characteristic concentration peaks in SW-620 and U87 cell lines, and provided in vivo tumor selectivity with several-fold higher tumor tissue fluorescence at the 6-h timepoint. Graphical abstract Fluorescent images of U-87 glioma cells after 24 hours of incubation with BSH-containing liposomes labeled with lipophilic Nile Red (red color)and water-soluble FITC-Dextran (green color); cell nuclei in blue color (DAPI-staining) (×400). Scale bar is 50 µm. Fluorescent labelling serves as anexpress method to study liposome delivery efficiency prior to boron accumulation evaluation and BNCT irradiation experiments.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Borohidruros / Colorantes Fluorescentes / Liposomas Tipo de estudio: Prognostic_studies Idioma: En Revista: J Fluoresc Asunto de la revista: BIOFISICA Año: 2021 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Borohidruros / Colorantes Fluorescentes / Liposomas Tipo de estudio: Prognostic_studies Idioma: En Revista: J Fluoresc Asunto de la revista: BIOFISICA Año: 2021 Tipo del documento: Article