A post-preservation vascular flush removes significant populations of donor leukocytes prior to lung transplantation.
Transpl Immunol
; 64: 101356, 2021 02.
Article
en En
| MEDLINE
| ID: mdl-33264679
ABSTRACT
BACKGROUND:
Donor leukocytes are intrinsically involved in acute lung allograft rejection, via self-presentation of donor antigens to recipient leukocytes. Therapeutic modalities to remove donor leukocytes are currently unavailable. We evaluated if a vascular flush immediately following preservation can be used for this purpose.METHODS:
A post-preservation flush was performed with STEEN solution in n = 6 porcine lungs following static cold storage. The first 500 ml effluent from the left atrium was collected and an inflammatory profile performed.RESULTS:
A total of 1.17 billion (±2.8 × 108) viable leukocytes were identified within the effluent. T cells were the dominant cell population, representing 82% of the total mobilised leukocytes, of which <0.01% were regulatory T cells. IL-18 was the most abundant cytokine, with a mean concentration of 84,216 pg (±153,552 pg). In addition, there was a mean concentration of 8819 ng (±4415) cell-free mitochondrial DNA.CONCLUSION:
There is an immediate transfer of donor leukocytes, cytokines and damage-associated molecular patterns following reperfusion. Such a pro-inflammatory donor load may enhance alloantigen presentation and drive recipient alloimmune responses. A post-preservation flush may therefore be an effective method for reducing the immune burden of the donor lung prior to transplantation.Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Preservación de Órganos
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Daño por Reperfusión
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Trasplante de Pulmón
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Linfocitos T Reguladores
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Leucocitos
Idioma:
En
Revista:
Transpl Immunol
Asunto de la revista:
ALERGIA E IMUNOLOGIA
/
TRANSPLANTE
Año:
2021
Tipo del documento:
Article