TINC- A Method to Dissect Regulatory Complexes at Single-Locus Resolution- Reveals an Extensive Protein Complex at the Nanog Promoter.
Stem Cell Reports
; 15(6): 1246-1259, 2020 12 08.
Article
en En
| MEDLINE
| ID: mdl-33296673
ABSTRACT
Cellular identity is ultimately dictated by the interaction of transcription factors with regulatory elements (REs) to control gene expression. Advances in epigenome profiling techniques have significantly increased our understanding of cell-specific utilization of REs. However, it remains difficult to dissect the majority of factors that interact with these REs due to the lack of appropriate techniques. Therefore, we developed TINC TALE-mediated isolation of nuclear chromatin. Using this new method, we interrogated the protein complex formed at the Nanog promoter in embryonic stem cells (ESCs) and identified many known and previously unknown interactors, including RCOR2. Further interrogation of the role of RCOR2 in ESCs revealed its involvement in the repression of lineage genes and the fine-tuning of pluripotency genes. Consequently, using the Nanog promoter as a paradigm, we demonstrated the power of TINC to provide insight into the molecular makeup of specific transcriptional complexes at individual REs as well as into cellular identity control in general.
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Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Complejos Multiproteicos
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Sitios Genéticos
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Células Madre Embrionarias Humanas
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Proteína Homeótica Nanog
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Stem Cell Reports
Año:
2020
Tipo del documento:
Article