High-throughput quantification of ochronotic pigment formation in <i>Escherichia coli</i> to evaluate the potency of human 4-hydroxyphenylpyruvate dioxygenase inhibitors in multi-well format.

Neuckermans, Jessie; Lequeue, Sien; Mertens, Alan; Branson, Steven; Schwaneberg, Ulrich; De Kock, Joery

High-throughput quantification of ochronotic pigment formation in Escherichia coli to evaluate the potency of human 4-hydroxyphenylpyruvate dioxygenase inhibitors in multi-well format.

Neuckermans, Jessie; Lequeue, Sien; Mertens, Alan; Branson, Steven; Schwaneberg, Ulrich; De Kock, Joery.

Afiliación

Neuckermans J; Department of In Vitro Toxicology and Dermato-Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.
Lequeue S; Department of In Vitro Toxicology and Dermato-Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.
Mertens A; Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany.
Branson S; Department of In Vitro Toxicology and Dermato-Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.
Schwaneberg U; Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany.
De Kock J; Department of In Vitro Toxicology and Dermato-Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.

MethodsX ; 8: 101181, 2021.

Article en En | MEDLINE | ID: mdl-33365261

Palabras clave

4-Hydroxyphenylpyruvate dioxygenase; Bacterial cell culture; Colorimetric; High-throughput screening; In Vitro; Inhibitor testing; Ochronotic pigment; Tyrosinemia

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