Establishment of Pancreatic Organoids from Normal Tissue and Tumors.
STAR Protoc
; 1(3): 100192, 2020 12 18.
Article
en En
| MEDLINE
| ID: mdl-33377086
ABSTRACT
Establishment of patient-derived adult stem cell-based pancreatic (tumor) organoids was first described in 2015. Since then, multiple laboratories have demonstrated the robustness of this method. We recently described the generation of a pancreatic cancer biobank containing 30 well-characterized tumor organoid models. Here, we describe the applied methods in detail. Use of tumor-selective media prevents contamination with normal cells. Generated organoids can be cryopreserved and can serve as a living biobank of pancreatic cancer for biomarker identification and drug screening. For complete information on the generation and use of this protocol, please refer to Driehuis et al. (2019).
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1
Base de datos:
MEDLINE
Asunto principal:
Páncreas
/
Organoides
/
Técnicas de Cultivo de Tejidos
/
Técnicas de Cultivo Tridimensional de Células
Idioma:
En
Revista:
STAR Protoc
Año:
2020
Tipo del documento:
Article