Single-Antibody Evaluation of T-Cell Receptor ß Constant Chain Monotypia by Flow Cytometry Facilitates the Diagnosis of T-Cell Large Granular Lymphocytic Leukemia.
Am J Clin Pathol
; 156(1): 139-148, 2021 06 17.
Article
en En
| MEDLINE
| ID: mdl-33438036
ABSTRACT
OBJECTIVES:
The diagnosis of T-cell large granular lymphocytic leukemia (T-LGLL) is challenging because of overlapping immunophenotypic features with reactive T cells and limitations of T-cell clonality assays. We studied whether adding an antibody against T-cell receptor ß constant region 1 (TRBC1) to a comprehensive flow cytometry panel could facilitate the diagnosis of T-LGLL.METHODS:
We added TRBC1 antibody to the standard T-cell and natural killer (NK) cell panel to assess T-cell clonality in 56 T-LGLLs and 34 reactive lymphocytoses. In addition, 20 chronic lymphoproliferative disorder of NK cells (CLPD-NKs) and 10 reactive NK-cell lymphocytoses were analyzed.RESULTS:
Clonal T cells were detected in all available T-LGLLs by monotypic TRBC1 expression and clonal/equivocal T-cell receptor gene rearrangement (TCGR) studies, compared with only 27% of T-LGLLs by killer-cell immunoglobulin-like receptor (KIR) restriction. Overall, 85% of T-LGLLs had a blood tumor burden greater than 500 cells/µL. Thirty-four reactive cases showed polytypic TRBC1 expression, except for 5 that revealed small T-cell clones of uncertain significance. All CLPD-NKs showed expected clonal KIR expression and negative TRBC1 expression.CONCLUSIONS:
Addition of TRBC1 antibody to the routine flow cytometry assay could replace the TCGR molecular study and KIR flow cytometric analysis to assess clonality, simplifying the diagnosis of T-LGLL.Palabras clave
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Base de datos:
MEDLINE
Asunto principal:
Receptores de Antígenos de Linfocitos T alfa-beta
/
Leucemia Linfocítica Granular Grande
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Anticuerpos de Cadena Única
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Citometría de Flujo
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Am J Clin Pathol
Año:
2021
Tipo del documento:
Article