Genome-wide CRISPR screening identifies TMEM106B as a proviral host factor for SARS-CoV-2.
Nat Genet
; 53(4): 435-444, 2021 04.
Article
en En
| MEDLINE
| ID: mdl-33686287
ABSTRACT
The ongoing COVID-19 pandemic has caused a global economic and health crisis. To identify host factors essential for coronavirus infection, we performed genome-wide functional genetic screens with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and human coronavirus 229E. These screens uncovered virus-specific as well as shared host factors, including TMEM41B and PI3K type 3. We discovered that SARS-CoV-2 requires the lysosomal protein TMEM106B to infect human cell lines and primary lung cells. TMEM106B overexpression enhanced SARS-CoV-2 infection as well as pseudovirus infection, suggesting a role in viral entry. Furthermore, single-cell RNA-sequencing of airway cells from patients with COVID-19 demonstrated that TMEM106B expression correlates with SARS-CoV-2 infection. The present study uncovered a collection of coronavirus host factors that may be exploited to develop drugs against SARS-CoV-2 infection or future zoonotic coronavirus outbreaks.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Genoma Humano
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Estudio de Asociación del Genoma Completo
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Sistemas CRISPR-Cas
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COVID-19
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Proteínas de la Membrana
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Proteínas del Tejido Nervioso
Tipo de estudio:
Diagnostic_studies
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Prognostic_studies
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Screening_studies
Idioma:
En
Revista:
Nat Genet
Asunto de la revista:
GENETICA MEDICA
Año:
2021
Tipo del documento:
Article