Pyroptosis inhibition alleviates potassium oxonate- and monosodium urate-induced gouty arthritis in mice.
Mod Rheumatol
; 32(1): 221-230, 2022 Jan 05.
Article
en En
| MEDLINE
| ID: mdl-33705241
ABSTRACT
OBJECTIVES:
Pyroptosis has been found implicated in several diseases, however, whether it was involved in gouty arthritis remained unclear. Our study was performed to uncover the role of pyroptosis in gouty arthritis based on a mice model.METHODS:
Mouse gouty arthritis model was established by injections of potassium oxonate (PO), monosodium urate (MSU) and pyroptosis suppressor disulfiram. The diameter of the ankle joints was measured, and ankle joints morphology was observed with hematoxylin-eosin (H&E) staining. Uric acid, creatinine and blood urea nitrogen (BUN) concentrations were measured, while cytokines level and xanthine oxidase (XOD) activity were quantified. Relative pyroptosis markers expressions were determined using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot as needed.RESULTS:
In mouse model, PO and MSU injections cause damage to right ankle, increase the root thickness ratio and uric acid, creatinine and BUN levels in serum and decrease the uric acid and creatinine levels in urine. Also, under PO and MSU treatment, up-regulated XOD activity, inflammatory cytokines levels and pyroptosis markers expressions are observed. Negative regulation of mice injury by disulfiram treatment is also observed.CONCLUSION:
Pyroptosis inhibition might alleviate PO- and MSU-induced gouty arthritis, providing possible therapeutic strategies for gouty arthritis.Palabras clave
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Base de datos:
MEDLINE
Asunto principal:
Artritis Gotosa
/
Piroptosis
Idioma:
En
Revista:
Mod Rheumatol
Año:
2022
Tipo del documento:
Article