Proteome Analysis with Classical 2D-PAGE.
Methods Mol Biol
; 2228: 53-62, 2021.
Article
en En
| MEDLINE
| ID: mdl-33950483
ABSTRACT
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) is based on the combination of two orthogonal separation techniques. In the first dimension, proteins are separated by their isoelectric point, a technique known as isoelectric focusing (IEF). There are two important variants of IEF, which are carrier-ampholine (CA)-based IEF and immobilized pH-gradient (IPG)-based IEF. In the second dimension, proteins are further separated by their electrophoretic mobility using SDS-PAGE. Finally, proteins can be visualized and quantified by different staining procedures such as Coomassie, silver staining, or fluorescence labeling. This article gives detailed protocols for 2D-PAGE, using both CA- and IPG-based separation in the first dimension.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Electroforesis en Gel Bidimensional
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Proteínas
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Proteoma
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Proteómica
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Electroforesis en Gel de Poliacrilamida
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Focalización Isoeléctrica
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2021
Tipo del documento:
Article