Mesoporous silica-mediated controllable electrochemiluminescence quenching for immunosensor with simplicity, sensitivity and tunable detection range.
Talanta
; 231: 122399, 2021 Aug 15.
Article
en En
| MEDLINE
| ID: mdl-33965049
ABSTRACT
Straightforward and accurate measurement of medical biomarkers is of essential importance in clinical diagnostics and treatments. However, the major challenge is the diversity in dynamic range of different biomarkers ranging from pg mL-1 to µg mL-1 in various body fluids and tissues among patients. Here, we develop a mesoporous silica (MS)-mediated controllable electrochemiluminescence (ECL) quenching of immunosensor that allows accurate immunoassays with simplicity, sensitivity and tunable sensing range. MS is employed to enhance the sensitivity and tune ECL quenching to broaden the detection range just by altering luminophore (Ru(bpy)32+) and coreactant (DBAE) concentration without additional modifications. The immunoassay is followed homogeneous sandwich immunoreaction, magnetic separation, and ECL quenching detection. As a proof-of-concept, simple and sensitive detection of IgG is achieved ranging from pg mL-1 to µg mL-1, and applications of the strategy are extended by the combination of ECL immunosensor with commercial ELISA kit. This study will not only be expected to serve as a new avenue for the assay of physiological and clinical implications of immunological biomarkers, but also benefit a wide range of applications that require a tunable detection range and ultrahigh sensitivity.
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Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Nanopartículas del Metal
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Talanta
Año:
2021
Tipo del documento:
Article