Rapid preparation of gastrointestinal nematode eggs from faeces for PCR identification.
J Microbiol Methods
; 187: 106257, 2021 08.
Article
en En
| MEDLINE
| ID: mdl-34062212
ABSTRACT
Detection of gastrointestinal nematodes (GIN) as both a qualitative and quantitative test is highly desirable. Methods such as multiplex and qPCR are capable of providing such results, but can be laborious and expensive. This paper presents a rapid, low-cost method of preparing GIN egg from faecal samples that produces DNA suitable for PCR analysis. We also describe a set of primers that are suitable for single-tube multiplex PCR.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Parasitología
/
Reacción en Cadena de la Polimerasa
/
ADN de Helmintos
/
Heces
/
Nematodos
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Infecciones por Nematodos
Tipo de estudio:
Diagnostic_studies
/
Qualitative_research
Idioma:
En
Revista:
J Microbiol Methods
Año:
2021
Tipo del documento:
Article