Assaying the Molecular Determinants and Kinetics of RNA Pseudouridylation by H/ACA snoRNPs and Stand-Alone Pseudouridine Synthases.

Czekay, Dominic P; Schultz, Sarah K; Kothe, Ute

Czekay, Dominic P; Schultz, Sarah K; Kothe, Ute.

Afiliación

Czekay DP; Department of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, Lethbridge, AB, Canada.
Schultz SK; Department of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, Lethbridge, AB, Canada.
Kothe U; Department of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, Lethbridge, AB, Canada. ute.kothe@uleth.ca.

Methods Mol Biol ; 2298: 357-378, 2021.

Article en En | MEDLINE | ID: mdl-34085255

Asunto(s)

Transferasas Intramoleculares/genética; Seudouridina/genética; ARN/genética; Ribonucleoproteínas Nucleolares Pequeñas/genética; Escherichia coli/genética; Cinética; Procesamiento Postranscripcional del ARN/genética; ARN Guía de Kinetoplastida/genética; Saccharomyces cerevisiae/genética

Palabras clave

Enzyme kinetics; H/ACA small ribonucleoprotein; Pseudouridine; Pseudouridine synthase; RNA modification tritium release assay

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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Seudouridina / ARN / Transferasas Intramoleculares / Ribonucleoproteínas Nucleolares Pequeñas Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2021 Tipo del documento: Article

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