Early Changes during Skin Repair Using Tissue-Engineered Dermal Template in a Full-Thickness Burn.
Folia Biol (Praha)
; 66(5-6): 161-168, 2020.
Article
en En
| MEDLINE
| ID: mdl-34087972
ABSTRACT
Rapid wound closure in extensively burned patients has remained one of the major unresolved issues of medicine. Integra® is the most widely established artificial skin, which is composed of a porous matrix of cross-linked bovine collagen and chondroitin 6-sulphate covered by a semi-permeable silicone layer. We present here a (immuno)histological study of a severely burned patient with a full-thickness burn treated with a tissue-engineered dermal template (Integra®) and split-thickness skin graft-based protocol. Immunohistochemical investigation of the artificial dermis revealed that immune cell infiltration reached its peak on day 10. Tissue immunophenotyping found an increase in CD3+ cells over the course of the study as well as CD4 and CD8 positivity on day 40, indicating remaining T-cell subpopulations. We observed weak/no infiltration of NK cells (CD56+). In conclusion, the use of bi-layer Integra® represents a feasible and safe procedure resulting in formation of non-irritating dermal substitutes.
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Base de datos:
MEDLINE
Asunto principal:
Quemaduras
/
Piel Artificial
Idioma:
En
Revista:
Folia Biol (Praha)
Año:
2020
Tipo del documento:
Article