Hoxa10 mediates positional memory to govern stem cell function in adult skeletal muscle.
Sci Adv
; 7(24)2021 06.
Article
en En
| MEDLINE
| ID: mdl-34108202
ABSTRACT
Muscle stem cells (satellite cells) are distributed throughout the body and have heterogeneous properties among muscles. However, functional topographical genes in satellite cells of adult muscle remain unidentified. Here, we show that expression of Homeobox-A (Hox-A) cluster genes accompanied with DNA hypermethylation of the Hox-A locus was robustly maintained in both somite-derived muscles and their associated satellite cells in adult mice, which recapitulates their embryonic origin. Somite-derived satellite cells were clearly separated from cells derived from cranial mesoderm in Hoxa10 expression. Hoxa10 inactivation led to genomic instability and mitotic catastrophe in somite-derived satellite cells in mice and human. Satellite cell-specific Hoxa10 ablation in mice resulted in a decline in the regenerative ability of somite-derived muscles, which were unobserved in cranial mesoderm-derived muscles. Thus, our results show that Hox gene expression profiles instill the embryonic history in satellite cells as positional memory, potentially modulating region-specific pathophysiology in adult muscles.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Células Madre
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Músculo Esquelético
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Proteínas Homeobox A10
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Mesodermo
Idioma:
En
Revista:
Sci Adv
Año:
2021
Tipo del documento:
Article