An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses.
Infect Genet Evol
; 93: 104967, 2021 09.
Article
en En
| MEDLINE
| ID: mdl-34116240
ABSTRACT
The real-time reverse transcription-polymerase chain reaction (real-time RT-qPCR) has become a leading technique for the detection and quantification of arboviruses, including Chikungunya, Dengue, and Zika viruses. In this study, an updated real-time RT-qPCR assay was designed and evaluated together with a synthetic positive-control chimeric RNA for the simultaneous detection and quantification of Chikungunya, Dengue, and Zika viruses. Amplification assays were performed to verify the construct integrity and optimal reaction/thermal cycling conditions. The analytical sensitivity of the assay was determined for each virus in single and multiplex reactions, as well as the performance in the detection and viral load quantification of experimental samples. The real-time RT-qPCR assay presented here allowed for the simultaneous detection and quantification of Chikungunya, Dengue, and Zika viruses and could be applied in several studies where the accurate quantification of viral genomes is required.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Virus Chikungunya
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Reacción en Cadena de la Polimerasa
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Virus del Dengue
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Pruebas Diagnósticas de Rutina
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Virus Zika
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Infect Genet Evol
Asunto de la revista:
BIOLOGIA
/
DOENCAS TRANSMISSIVEIS
/
GENETICA
Año:
2021
Tipo del documento:
Article