Protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines.
STAR Protoc
; 2(3): 100495, 2021 09 17.
Article
en En
| MEDLINE
| ID: mdl-34195669
ABSTRACT
During eukaryotic cell mitosis, the nuclear envelope disintegrates and transcription factors are dissociated from condensed chromosomes. Here, we describe a protocol to study centrosomal translocation of nuclear receptor RXRα. We detail procedures for HeLa cell synchronization followed by immunofluorescence, in situ proximity ligation assay, and centrosome isolation. This protocol can be used to identify other transcription factors associated with the centrosome or other subcellular structures during mitotic progression. For complete details on the use and execution of this protocol, please refer to Xie et al. (2020).
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
/
Centrosoma
/
Mitosis
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
STAR Protoc
Año:
2021
Tipo del documento:
Article