Oat Doubled Haploid Production Through Wide Hybridization with Maize.
Methods Mol Biol
; 2287: 323-332, 2021.
Article
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| MEDLINE
| ID: mdl-34270040
ABSTRACT
Wide hybridization is one of the haploid-inducing techniques that can accelerate the breeding process. Obtaining new cultivars is crucial to solve the problem of the constantly growing world population and global increase in demand for food, feed and renewable energy under changing environmental conditions. Here, we present a detailed protocol for obtaining oat (Avena sativa L.) doubled haploids (DHs) by pollination with maize (Zea mays L.). After fertilization, not only oat homozygotes, but also oat × maize hybrid zygotes can be formed, and during early embryo development, maize chromosomes are preferentially eliminated, which ultimately results in haploid plant formation. This chapter describes a method to produce oat DHs by crossing oat with maize, covering all steps from crossings to haploid plant regeneration and chromosome doubling.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Avena
/
Zea mays
/
Hibridación Genética
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2021
Tipo del documento:
Article