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Circulating miR-146a as a possible candidate biomarker in the indeterminate phase of Chagas disease.
Ballinas-Verdugo, Martha Alicia; Jiménez-Ortega, Rogelio Frank; Martínez-Martínez, Eduardo; Rivas, Nancy; Contreras-López, Erick Abraham; Carbó, Roxana; Sánchez, Fausto; Bojalil, Rafael; Márquez-Velasco, Ricardo; Sánchez-Muñoz, Fausto; Alejandre-Aguilar, Ricardo.
Afiliación
  • Ballinas-Verdugo MA; Departamento de Inmunología, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, CDMX, Mexico. ballinasv75@gmail.com.
  • Jiménez-Ortega RF; Departamento de Parasitología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, CDMX, Mexico. ballinasv75@gmail.com.
  • Martínez-Martínez E; Licenciatura en Nutrición, Plantel Texcoco, Universidad Privada del Estado de México, Texcoco, Estado de México, Mexico.
  • Rivas N; Instituto Nacional de Medicina Genómica, Mexico City, CDMX, Mexico.
  • Contreras-López EA; Departamento de Parasitología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, CDMX, Mexico.
  • Carbó R; Instituto Mexicano del Seguro Social, Hospital General Regional No. 25 Zaragoza, Mexico City, CDMX, Mexico.
  • Sánchez F; Departamento de Biomedicina Cardiovascular, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, CDMX, Mexico.
  • Bojalil R; División de Ciencias Biológicas y de La Salud, Universidad Autónoma Metropolitana Xochimilco, Mexico City, CDMX, México.
  • Márquez-Velasco R; División de Ciencias Biológicas y de La Salud, Universidad Autónoma Metropolitana Xochimilco, Mexico City, CDMX, México.
  • Sánchez-Muñoz F; Departamento de Inmunología, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, CDMX, Mexico.
  • Alejandre-Aguilar R; Departamento de Inmunología, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, CDMX, Mexico.
Biol Res ; 54(1): 21, 2021 Jul 21.
Article en En | MEDLINE | ID: mdl-34289913
ABSTRACT

BACKGROUND:

Chagas disease is considered important and presents intense inflammatory and fibrotic processes induced by the perpetuation of the parasite in the affected tissues and organs. Therefore, it is necessary to inquire about the host defense and attack mechanisms to have a more detailed knowledge about Chagas disease. MicroRNAs are found in blood, tissues and extracellular vesicles. These small regulators of gene expression are involved in physiological and pathological processes in both mammals and parasites. Several microRNAs have deregulated expression in chagasic heart disease, although little is known about their extracellular expression. Our main objective was to evaluate the involvement of miR-21, miR-146a and miR-155 in several samples from mice infected with the TcI Ninoa strain from the acute and indeterminate phases. We also explored a potential functional association of the selected microRNAs using STRING software. This software identified 23 pathways associated with Trypanosoma cruzi infection. In addition, eleven genes were identified through bioinformatics analysis, and we found that SMAD family member 5 was downregulated in both phases. This gene serves as a mediator in the TGF-ß signaling pathway. Thus, forty female mice of the CD1 strain were distributed into 4 groups and the expression levels of miR-21, miR-146a and miR-155 were measured in samples of heart tissue, total plasma and plasma extracellular vesicles by quantitative real-time polymerase chain reaction.

RESULTS:

Overexpression of miR-21, miR-146a and miR-155 was observed in heart and plasma in both phases. Moreover, in extracellular vesicles miR-21 and miR-146a were also overexpressed in the acute phase, whereas in the indeterminate chronic phase we found only miR-146a up-regulated.

CONCLUSIONS:

The expression of inflammatory microRNAs miR-21, miR-146a and miR-155 were up-regulated in each of the samples from acutely and chronically infected mice. The relevant finding was that miR-146a was up-regulated in each sample in both phases; therefore, this miRNA could be a possible candidate biomarker in Chagas disease.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Enfermedad de Chagas / MicroARNs Tipo de estudio: Prognostic_studies Idioma: En Revista: Biol Res Asunto de la revista: BIOLOGIA Año: 2021 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Enfermedad de Chagas / MicroARNs Tipo de estudio: Prognostic_studies Idioma: En Revista: Biol Res Asunto de la revista: BIOLOGIA Año: 2021 Tipo del documento: Article