Label-free methods for optical in vitro characterization of protein-protein interactions.
Phys Chem Chem Phys
; 23(31): 16488-16500, 2021 Aug 12.
Article
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| MEDLINE
| ID: mdl-34342317
ABSTRACT
Protein-protein interactions are involved in the regulation and function of the majority of cellular processes. As a result, much effort has been aimed at the development of methodologies capable of quantifying protein-protein interactions, with label-free methods being of particular interest due to the associated simplified workflows and minimisation of label-induced perturbations. Here, we review recent advances in optical technologies providing label-free in vitro measurements of affinities and kinetics. We provide an overview and comparison of existing techniques and their principles, discussing advantages, limitations, and recent applications.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Proteínas
Idioma:
En
Revista:
Phys Chem Chem Phys
Asunto de la revista:
BIOFISICA
/
QUIMICA
Año:
2021
Tipo del documento:
Article